Identification of IgE-binding protein from mandarin fish and antigenicity reduced by proteinases hydrolysis

SDS-PAGE was employed to further identify the relative contents of major allergens of mandarin fish(Siniperca chuatsi) protein.On the basis of positive serum,transfer electrophoresis,western blotting and immunoinhibition tests were used to quantify specific adsorption of protein bands.And then six different sources of food-grade proteases were adopted to hydrolyze allergens in order to hyposensitization.As two indicators of the optimized hydrolase,degree of hydrolysis and lower rates of allergens were checked by Neutral formaldehyde titration and indirect enzyme-linked immunosorbent assay.Finally the molecular weight of 60 ku,5 ku,3 ku and 1 ku protein bands can be connected with the specific immunoglobulin E.Secondly the best enzyme was papain.