Preparation and application of Fmoc- D phenylalanine/hyaluronic acid composite double-network hydrogel

N-fluoren-methoxycarbonyl-D-Phenylalanine (Fmoc-DPhe) and Glycidyl Methacrylate (GMA)-modified hyaluronic acid (HA-GMA) were mixed and heated in phosphoric acid buffer. After cooling, Fmoc-DPhe molecules were self-assemble into supermolecular hydrogel followed by forming double network hydrogel with HA-GMA under UV light irradiation. The mechanical properties, optical properties, microstructure, drug release and bacteriostatic properties of the composite hydrogel were studied. The results show that the double-network hydrogels are about twice as strong as those of HA-GMA single-network hydrogel, and the HA-GMA network exists in the double-network hydrogels. Optical properties show the existence of Fmoc-DPhe network in the double network hydrogels. The microstructure indicated that there were two kinds of hydrogel networks in the composite hydrogel. Moreover, after loading the drug, the double-network hydrogel took 6 hours longer to reach the maximum drug accumulation rate than the Fmoc-DPhe single-network hydrogel. The antibacterial activity of the double-network hydrogel against gram-positive bacteria was also better than that of the Fmoc-DPhe single-network hydrogel.