Biomimetic Gastrointestinal Tract Functions for Metal Absorption Assessment in Edible Plants: Comparison to In Vivo Absorption

A biomimetic gastrointestinal tract, including in vitro digestion and biomimetic biomembrane extraction, has been proposed for absorption assessment of metals from edible plants. However, its validity is still unknown. Herein, two species of edible plants, Anoectochilus roxburghii and Radix astragali, were selected and digested in a bionic mouth, stomach, and intestine, and then trace metals (Cr, Mn, Fe, Ni, Cu, Zn, Se, Sr, As, and Pb) were transformed to their final metal species. To check model predictability, in vitro and in vivo metal absorption were imitated and tested by monolayer liposome extraction and rat stomach or single-pass duodenal intestine, respectively. A strong correlation was established between in vivo and in vitro metal absorption ratios, with 0.89 > R2 > 0.66, and a significant relationship (p < 0.05) was exhibited for stomach, intestine, two plant species, and 10 metal species. Our biomimetic system could be used as low-cost alternatives to animal and clinical studies for multi-meta...