Immobilized ficin catalyzed synthesis of peptides in organic solvent

Abstract Immobilized ficin catalyzed the synthesis of peptides between N -protected amino acids and amino acid esters in ethyl acetate. Under similar conditions N,N′- diBoc- l -cystine was furnished the monopeptidation by controlling the duration of the reaction. The influence of pH, reaction time and amount of triethylamine on mono-peptidation was also studied.