HERCEPTEST :HER 2EXPRESSIO NAN DGEN EAMPLIFICATION I NNON-SMAL LCEL LLUN GCANCER

HER 2i sa nerbB/HE Rtyp e 1tyrosin ekinas erecepto rthat i sfrequentl yover-expresse di nmalignan tepithelia ltumours. Herceptin ,a humanise dmous emonoclona lantibod yto HER2 ,i sprove ntherapeuticall yi nth emanagemen to fmetastati cbreas tcancer ,significantl yprolongin gsurviva lwhen combine dwit hcytotoxi cchemotherapeuti cagents .Immunohistochemica lstudie ssugges ttha tnon-small-cel llun gcance r(NSCLC )tumour sma yover-expres sHER2 .Ou rai mwas t oevaluat eHER 2gen eamplificatio nan dsemi-quantitative immuno-expressio ni nNSCLC .A tota lo f34 4NSCL Ccases wer eimmunostaine dfo rHER 2expressio ni n2 centre susing th eHercepTest .Fluorescenc ei nsit uhybridisatio n(FISH) analysi sfo rHER 2gen eamplificatio nwa sperforme do nmost positiv ecase san da subse to fnegativ ecases .Fiftee ncases (4.3% )demonstrate d2 1o r3 1membranou sHER 2immunoexpression .Ther ewa sn ocorrelatio nbetwee nimmuno-expressio nan dtumou rhistolog yo rgrade .Tumour sfro mhigher-stag ediseas ewer emor eofte nHercepTest-positiv e( p< 0.001) .Al l4 HercepTes t3 1 case sdemonstrate dgen eamplification .On eo fth e 52 1case steste dfo rgen eamplification showe darea so fborderlin eamplificatio nan darea sof polyploidy .Non eo fth e1 9HercepTest-negativ ecase sdemonstrate dgen eamplificatio no rpolyploid y( p <0.001) .Gene amplificatio nwa sdemonstrate di nal lHercepTes t3 1scoring NSCL Ccases .Unlik ebreas tcancer ,gen eamplificatio nand HER 2protei nover-expressio nassesse db yth eHercepTest appeare dt ob euncommo ni nNSCLC .Hercepti nma ytherefor etarge tonl ya smal lproportio no fNSCL Ctumour sand b eo flimite dclinica lvalu ei nthi sdisease ,particularl yi nthe adjuvan tsetting.