Amperometric Detection of Antioxidant Activity of Model and Biological Fluids

The reactive oxygen species (ROS) generated during the course of many metabolic processes are capable of oxidizing biologically active compounds (BAC). The total water-soluble antioxidant contents (TWSAC) in the model and biological fluids are determined with the amperometric method (TsvetYauza-01-AA chromatography device, Russia). Thus, the TWSAC for biologically active ascorbic acid, sea buckthorn, white grapes, and amino acids (AAs) comprised 46.53, 0.54, 0.28, and 4.00 mg/g, respectively (as the average value for their blood content). The TWSAC in the blood sera of cattle in different age and sex groups, kept under comparable experimental conditions, ranged from 14.8 to 21.3 mg/g. The water-soluble antioxidant level in youngstock (3 months old) was significantly higher than that in lactating cows, which is probably due to the higher serum concentrations of aromatic AAs having powerful antioxidant properties in the young specimens. The datasets generated during the current study are important in assessing the physiological and biochemical status and evaluating the efficiency of the antioxidant defense systems in the body of an animal.