Studies on the constituents of Pinus armandii with xanthine oxidase inhibitory activities

Objective To study the chemical constituents of the knobs of Pinus armandii and their xanthine oxidase inhibitory ac-tivities .Methods Compounds were isolated and purified by using various chromatographic technologies and their structures were identified on the basis of physicochemical properties and spectroscopic data .The xanthine oxidase inhibitory activities were deter-mined by UV spectrophotometric method .Results Eight compounds were identified as isopimara-7-en-18-oic acid (1) ,lambertian-ic acid (2) ,3β,21α-dimethoxyserrat-14-ene (3) ,3β-methoxyl-21α-acetoxyserrat-14-ene (4) ,3β-methoxyserrat-14-en-21-one (5) ,β-sitosterol (6) ,cholesteryl myristate (7) and docosanoic acid (8) .Conclusions Compound 3 was isolated from this plant for the first time ,and also its 13C-NMR data were reported for the first time .Compound 1 and 2 showed some xanthine oxidase inhibitory activities .