RAPD Analysis of Germplasm Resources of Kudingcha in Oleaceae

【Objective】The genetic difference and relationship among germplasm resources of Kudingcha in Oleaceae were revealed at molecular levels on this study,which could be considered as the significant reference bases for the classification and identification and breeding of Kudingcha species in Oleaceae.【Method】The random amplified polymorphic DNA marker(RAPD) was applied to detect the genetic relationships and diversity among 21 germplasm materials of Kudingcha in Oleaceae,which involved in 8 species,i.e.Ligustrum robustum(Roxb.) Blume,L.henryi Hemsl.,L.japonicum Thunb.,L.japonicum Thunb.var.pubscens Koidz,L.lucidum Ait.,L.pedunculare Rehd,Osmanthus masumuranus Hayata,L.delavayanm Hariot.The Jaccard similarity coefficient of 21 germplasm materials was calculated,and the UPGMA was used for cluster analysis.【Result】20 RAPD primers selected were applied for the amplification on the 21 germplasm materials mentioned above.427 bands were obtained,and the percentage of polymorphic bands(PPB) was 97.7%.The genetic similarity coefficients(GS) ranged from 0.1522 to 0.8322 with an average of 0.5466.There were significant genetic difference among germplasm materials of Kudingcha species in Oleaceae,and UPGMA cluster based on GS of RAPD could distinguish all test germplasm materials clearly and indicated relationship of 8 species mentioned above.【Conclusion】RAPD markers could be used for the studies of genetic diversity and relationship and classification of germplasm resources of Kudingcha in Oleaceae.Analysis results of RAPD showed that L.japonicum Thunb.var.pubscens Koidz has closer genetic relationship with L.pedunculare Rehd and further genetic relationship with L.japonicum Thunb.among all tested species.The authors suggested that the further research be needed to study whether L.japonicum Thunb.var.pubscens Koidz should be classified into a variata of L.japonicum Thunb.,or considered as an independent species.The analysis results supported that L.pururascens Y.C.Yang should be combined into L.robustum(Roxb.) Blume.