Antioxidant Activity of Isolated Compounds from the Shoot of Aralia elata Seem

ABSTRACT Objectives : This study was performed to investigate the antioxidant activi ties of isolated compounds from the shoot of Aralia elata.Methods : The methanol extract from the shoot of Aralia elata was fractionated into ethyl acetate, n-BuOH and H 2 O layers through solvent fractionation. Repeated silica gel, ODS column chromatography of n-BuOH layer afforded four flavonol glycosides. Their antioxidan t activity was determined by measuring free radical scavenging activity by DPP H, ABTS and superoxide dismutase (SOD) like activity assay. Results : They were identified as quercetin 3,7-di- O-α-rhamnopyranoside (1), quercetin 3-O-β- D-galactoside-7-O-α-L-rhamnoside (2), kaempferol 3- O-β-glucosyl(1→2)-α-rhamnoside-7-O-α- rhamnoside (3) and quercetin 3-O-β-glucosyl(1→2)-α-rhamnoside-7-O-α-rhamnoside (4) on the basis of spectroscopic data. The result showed that 1 is the most active compound in the DPPH and ABTS radical scavenging test. Conclusions : Isolated Compounds from the shoot of Aralia elata showed anti-oxidative effect. Key words : Aralia elata, antioxidant activity, flavonol glycosides