Purification and properties of RNA polymerases I and II from germinated spores of Rhizopus stolonifer

RNA polymerases I and II were purified several hundredfold from germinated spores of the mycelial fungus, Rhizopus stolonifer. Three major subunit proteins with estimated molecular weights of 180 000, 104 000, and 32 000 daltons were obtained from RNA polymerase I and four major subunit proteins with estimated molecular weights of 215 000, 114 000, 37 000, and 27 000 daltons were obtained from RNA polymerase II. Therefore, the molecular structures of RNA polymerases I and II from R. stolonifer are essentially similar to those purified from other eukaryotic organisms.