Галактоза способствует размножению бактерий псевдотуберкулеза в условиях in vitro и in vivo

Summary The authors study effects of galactose in reproduction of enterobacteria and virulent properties of pseudotuberculosis bacteria of galactose phenotype. Twenty strains of enterobacteria were being cultivated under aerobic conditions using Luria broth (LB) and LB with 0.5 % of galactose (LB+Gal). The reproduction dynamics was estimated by studying optical density of suspensions. Noninbred mice were infested with Yersinia pseudotuberculosis strain known to carry recombinant plasmid with reporter system based upon fluorescent protein. The authors have studied two groups by 10 animals undergone peroral introduction of bacterial suspension cultivated in LB and LB+Gal broths. The biological material was inoculated on nutrient agar with chloramphenicol to calculate the number of colonies per 1 gram of fecals mass and liver in 48-72 hours. Most of Y. pseudotuberculosis strains were propagating more intensively in the LB+Gal broth. The others, except Y. ruckeri and Y. pestis Pestoides, showed the same characteristics. The strains of Escherichia coli found in the LB+Gal broth demonstrated increasing multiplication. The Salmonella and Shigella strain propagation did not depend on carbohydrates. As observed, Y. pseudotuberculosis bacteria cultivated in the LB+Gal broth propagated more intensively in the digestive tract and demonstrated increasing capability of penetrating epithelial barrier of bowels thus percolating the internal environment.