Preparation of five phenylethanoid glycoside components by semi-preparative HPLC.

A method was established for the isolation and preparation of five phenylethanoid glycoside components from Chirita eburnea using MCI column chromatography and semi-preparative HPLC.A semi-preparative Eclipse XDB-C18 (9.4 mm×250 mm,5 μm) was used in the preparation process.By gradient elution with methanol-water as the mobile phase at a flow rate of 4 mL/min,five phenylethanoid glycoside components were obtained from Chirita eburnea,and on the basis of spectral data (EI-MS,1H-NMR,13C-NMR),these compounds were identified as plantainoside(1),chiritoside C(2),plantaninoside B(3),plantamajoside(4) and desrhamnosylverbascoside(5),and the purities of these five phenylethanoid glycoside components analyzed by HPLC were all beyond of 98%.The method is efficient,simple with high purification and got 5 components for one time.