Study on HPLC-FPS of Saponins from Pairs polyphylla Smith var yunnanensis(Franch)Hara

[Objective] The research aimed to establish a method of HPLC-fingerprint spectrum(HPLC-FPS) for the active part in Pairs polyphylla Smith var yunnanensis(Franch)Hara in order to control it's quality from different palaces.[Method] The isocratic elution mode was applied in chromatographic separation,and data were analysed by "Similarity Evaluation software" to compare it's HPLC-FPS from different places.[Result] The conditions for HPLC analysis of Pairs polyphylla Smith var yunnanensis(Franch)Hara were established and 8 common peaks were separated.[Conclusion] All components in the spectrum were separated well and the HPLC fingerprint method is repeatable.The method can be used in quality assessment of Pairs polyphylla Smith var yunnanensis(Franch)Hara