СРАВНЕНИЕ ДИАГНОСТИЧЕСКОЙ ЭФФЕКТИВНОСТИ МЕТОДОВ ДЕТЕКЦИИ COXIELLA BURNETII В КРОВИ БОЛЬНЫХ ЛИХОРАДКОЙ КУ НА ОСНОВЕ АМПЛИФИКАЦИИ ФРАГМЕНТОВ ГЕНА 16S рРНК (СТАНДАРТНАЯ ПЦР) И ГЕНА groEL (ПЦР В РЕЖИМЕ РЕАЛЬНОГО ВРЕМЕНИ)
2016
Aim. Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burnetii persistence in dynamics of infectious process in patients with Q fever. Materials and methods. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time PCR (RT-PCR) (marker - groEL gene fragment). Results. Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). Conclusion. This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.
Keywords:
- Correction
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI