The effect of esomeprazole use to bone mineral density and bone metabolism of male rats

Objective This study aimed to investigate the effects of different doses of esomeprazole use of male SD rats bone mineral density and bone metabolism at different time points in order to guide clinical treatment.Methods Twenty four 3-month-old male rats were divided into three groups:the control group received the vehicle only,the low-dose esomeprazole group was treated with esomeprazole of 10 mg/ (kg·d) and the high-dose esomeprazole group was treated with esomeprazole of 50 mg (kg· d).We assess total bone mineral densities (BMDs) and bone alkaline phosphatase (B-ALP),tartate resistant acid phosphatase 5b (TRACP 5b) and serum calcium concentration at weeks 0,8 and 14.Bone histomorphometric analysis was performed to evaluate the structural changes in the femur of rats after sacrifice.Results The body weight of the high-dose esomeprazole group was suppressed whereas that of the control group increased significantly at week 8 (P ＜ 0.05).The BMD of the high-dose group decreased dramatically whereas that of the other two groups increased significantly at week 14.Serum B-ALP,TRACP 5b and calcium concentrations increased significantly which is (198.51 ± 6.98),(1.49 ± 0.04) U/L and (2.44 ± 0.05) mmol/L respectively.Significant changes in the results were not observed at week 8 (P ＞ 0.05).Bone histomorphometric analysis showed significantly different bone structures among the three groups at week 14 and the ER of the high-dose group was significantly higher than the control group and thelow-dose group.Conclusion Long term and high dose use of esomeprazole reduces bone mineral density and effects bone metabolism of male rats in a time-dependent manner. Key words: Esomeprazole;  Bone mineral density;  Bone metabolism;  Bone histomorphometric analysis;  Osteoporosis