HPLC method for rapidly detecting main active ingredients of lithocarpus polysachyus rehd

The invention discloses an HPLC method for rapidly detecting main active ingredients of lithocarpus polysachyus rehd. The method comprises treatment of a measured sample, extraction of the measured sample, preparation of a standard sample and detection. The method comprises the specific steps: selecting lithocarpus polysachyus rehd powder with the granularity of 60-120 meshes as a raw material sample, performing ultrasonic extraction on the powder by adopting an aqueous solution of methanol, repeatedly extracting for 4 times, merging the supernatant, filtering the supernatant by using a 0.45mu m microfiltration membrane, and analyzing and measuring the filtrate by using a high performance liquid chromatograph, wherein a mobile phase of HPLC consists of acetonitrile and a 0.02-0.06 volume percent of aqueous solution of formic acid according to a volume ratio of 26-30:70-74, isocratic elution is performed, the flow velocity is 0.7-0.9mL.min , and the detection wavelength is 285nm. The method can intuitively and rapidly evaluate the quality of wild lithocarpus polysachyus rehd and provides a scientific basis for selecting a good variety of lithocarpus polysachyus rehd and directionally breeding the lithocarpus polysachyus rehd.