[Protection mechanisms of ATP-sensitive K channels on hippocampal CA1 neurons during chronic severe hypoxia].

Aim:To study the protection mechanisms of KATP channels on hippocampal CA1 neurons during chronic severe hypoxia.Methods:p53 expression,DNA fraction,and cell apoptosis were examined in cultured hippocampal neurons in control group,hypoxia group,hypoxia group treated with KATP channels antagonist and hypoxia group treated with KATP channels agonist.Results:In the group of a 12 h long exposure to oxygen concentration of 0%,diazoxide(100 μmol/L),the KATP channels agonist,reduced p53 expression and the hypoxia-induced apoptosis.In contrast,tolbutamide(100 μmol/L),the KATP channels antagonist,significantly rose p53 expression and the hypoxia-induced apoptosis,which could be reversed by p53 inhibitor TSA.Conclusion:KATP channels protect hippocampal neurons against chronic severe hypoxia by suppressing p53 expression.