An isoelectrofocusing study of rabbit pyruvate kinases

Abstract 1. 1. Extracts of rabbit thigh muscle, liver, kidney cortex and spleen were subjected to isoelectrofocusing. In some cases isolated activity peaks were refocused. 2. 2. Evidence for six variant forms of the K-isozyme were obtained. They had pI values of ~5.4, ~ 5.9, 7.0, 7.4, 8.3 and 8.8. Evidence was also obtained for the existence of two L-isozyme variants with pI values of 4.7 and 5.0. The M-isozyme had a pI value of 8.9. 3. 3. These data were compared to isoelectrofocusing data obtained for other vertebrate species. The pleomorphism of the K and L-isozymes appears to be the rule.