Desenvolvimento de técnicas de bioanálise in vitro para monitoração individual de 32P

The 32 P is used in the form of liquid unsealed sources in medical facilities, research and teaching, representing a risk of internal exposure in routine activities and in case of accidental incorporation. The evaluation of 32 P incorporation can be accomplished through in vitro bioanalysis of urine. This paper aims to provide a methodology to analyze 32 P in biological samples, applicable to internal individual monitoring using liquid scintillation technique. The minimum detectable activity of the system was determined and the sensitivity of the technique was evaluated, based on the detected minimum effective dose.