[Construction of a stable genetically engineered microorganism for degrading HCH & methyl parathion and its characteristics].

A GEM designated as BHC-A-mpd,capable of simultaneously degrading of methyl parathion(MP) and HCH was successfully constructed by random insertion of a methyl parathion hydrolase gene(mpd) into chromosome of a HCH-degrading strain BHC-A with the mini-Tn-transposon system.The growth and degrading characteristics of BHC-A-mpd was compared with the original strain BHC-A,and the result showed that there was no difference in this two aspects,A_(600 nm) of BHC-A-mpd in LB medium could reach 2.5 in logarithmic period,which was the same as that of the original strain BHC-A.BHC-A-mpd showed the same HCH-degrading ability as BHC-A and could degrade 5(mg/L) of(γ-HCH) in(10 h).BHC-A-mpd showed high genetica stability and could degrade many kinds of organophosphorus pesticides.All these results indicated that BHC-Ampd was a promising GEM in bioremediation of MP and HCH co-contaminated environment.