The Growth Inhibitory Effect of Ad-ING4 on Human Hepatocarcinoma Cell Line SMMC-7721

Objective To investigate the effect of adenovirus-mediated ING4 gene ( Ad-ING4) on human hepatocarcinoma cell line SMMC-7721 in vitro. Methods After the Ad-ING4 was transfected into SMMC-7721 cells,we used RT-PCR and indirect immunofluorescence to confirm that ING4 gene transcripted and expressed in SMMC-7721 cells. Fluorescent microscopy was used to observe the cytotoxicity of Ad-ING4 to SMMC-7721 cells. The proliferation curve of SMMC-7721 cells was drawn based on MTT assay,and the inhibitory rate was calculated. Cellular Apoptosis of SMMC-7721 cells induced by Ad-ING4 was detected by FCM. DAPI staining was used to observe the morphological changes of the nuclei. Results The transcription and expression of ING4 gene in SMMC-7721 cells was detected by RT-PCR and indirect immunofluorescence. Seventy-two hours and ninety-six hours after infecting with Ad-ING4,the growth inhibitory rate was 33. 82% and 50. 70% respectively. The apoptosis peak was found by FCM and the rate could reach 46. 7% . The classical morphological changes of the nuclei were shown by DAPI staining such as anachromasis,karyopycnosis,breakage and so on. Conclusion Ad-ING4 could significantly inhibit the proliferation of SMMC-7721 cells,and induce the cells to apoptosis.