A Rabbit Model of Cardiopulmonary Bypass under Deep Hypothermia Circulatory Arrest

Objective To establish the model of cardiopulmonary bypass(CPB) under deep hypothermia circulatory arrest(DHCA) in the rabbits.Methods 10 health rabbits weighting 2.5±0.5 kg were used in this study.A 16-gauge trocar was inserted directly as an aortic cannula,and a metallic plug of the venous cannula was inserted into the right auricular appendage to drain venous blood.Deep hypothermic circulatory arrest lasted for 60 minutes.After systemic cooling and the establishment of a hypothermic rectal temperature of 20℃,DHCA was induced for 60 minutes.The animals were then reperfused and rewarmed to achieve a normal rectal temperature of 36℃.Electrocardiogram and hemodynamics were monitored in the total procedure.Results The model of CPB under DHCA was successfully established in 9 rabbits(one rabbit died of bleeding in the surgery).The primary volume of fluid was 75 mL,and the CPB circuit was 140 minutes on average.Mean arterial pressure was within normal limits.Conclusion Rabbit model of CPB under DHCA was feasible and stable to research pathophysiological and pathological changes during CPB and the effect and mechanism of protection of cerebrum function.