Ultrastructural effects of pressure stress to Saccharomyces cerevisiae cells revealed by immunoelectron microscopy using frozen thin sectioning

Abstract Effects of hydrostatic pressure on ultrastructure of Saccharomyces cerevisiae were studied by immunoelectron microscopy using frozen thin sections. At 100 MPa bundles of the microtubules (MTs) extended in the nucleus, but spindle pole bodies were not visible. At 150 MPa the deposition of gold particles for anti α-tubulin was recognized in the nucleus, although the filamentous structure of the MTs was not seen. At 200 MPa fewer gold particles were scattered in the nucleus. These results show that elements of the nuclear division apparatus are susceptible to pressure stress. These events were reversible at below 200 MPa.