Development of an HPLC-MS/MS Method for Quantitative Determination of Rivaroxaban in Human Blood Serum

An HPLC-MS/MS method for determination of rivaroxaban in human blood serum was developed. Sample preparation included protein precipitation by MeOH followed by centrifugation and dilution with deionized H2O. The developed procedure had simple sample preparation, a short analysis time, and a wide analytical range (1.00 to 1,000.00 ng/mL). The procedure was convenient for routine bioanalytical studies, in particular, for therapeutic drug monitoring.