Innovative hybrid system for wastewater treatment: High-rate algal ponds for effluent treatment and biofilm reactor for biomass production and harvesting

Abstract The use of algal biomass still faces challenges associated with the harvesting stages. To address this issue, we propose an innovative hybrid system, in which a biofilm reactor (BR) operates as an algal biomass production and harvesting unit connected to a high-rate algal pond (HRAP), a wastewater treatment unit. BR did not interfered with the biomass chemical composition (protein = 32%, carbohydrates = 11% and total lipids = 18%), with the wastewater treatment (removals efficiency: chemical oxygen demand = 59%, ammonia nitrogen = 78%, total phosphorus = 16% and Escherichia coli = 1 log unit), and did not alter the sedimentation characteristics of the biomass (sludge volume index = 29 mg/L and humidity content = 92%) in the secondary settling tank of the hybrid system. On the other hand, the results showed that this technology achieved a biomass production about 2.6x greater than the conventional system without a BR, and the efficiency of harvesting of the hybrid system was 61%, against 22% obtained with the conventional system. In addition, the BR promoted an increase in the density (~1011 org/m2) and diversity of microalgae in the hybrid system. Chlorella vulgaris was the most abundant species (>60%) from the 4th week of operation until the end of the experiment. Hence, results confirm that the integration of BR into a wastewater treatment plant optimised the production and harvesting of biomass of the hybrid system, making it a promising technology. The importance of economic and environmental analysis studies of BR is highlighted in order to enable its implementation on a large scale.