Immune Effect of Biodegradable Polymer Microparticle Vaccine Against Dental Caries by Oral Administration

[Objective]The peptide derived from Pac (301~319) was selected as antigens. PBLG-PEG-PBLG block copolymer was selected as carrier to prepare microparticle vaccine which can hold PAc peptide (301~319). Then we investigated the colonization of Streptococcus mutans on experimental rat tooth surface and the effect of oral administrated vaccine against rat dental caries. [Methods] Twenty-eight Sprague-Dawley gnotobiotic rat models were randomly divided into 4 groups. The colonization numbers of S. mutans were counted and the presence of caries was quantified by the Keyes scoring system.[Results] The bacteria numbers of the PAc peptide microparticle group were (13.2±8.16)×104 CFU/ml and the Keyes scores were 31.8±6.77. Both were significantly lower than those of the control group(P 0.01). [Conclusions] The PAc peptide micropaticle vaccine by oral administration could reduce the development of experimental rat caries, especially the caries on tooth smooth surface.