Application of enantioselective separation techniques to bioanalysis of chiral drugs and their metabolites

Abstract Separations of enantiomers are performed for very different purposes. Although the basic approach to separate enantiomers can be quite general, there may be significant differences in method refinement based on the final application. This short overview deals with the specific requirements that the bioanalytical applications pose to the enantioseparation methods, compares various separation methods from the viewpoint of their suitability and potential for bioanalytical purposes and discusses current developments, unresolved problems and future trends in this field.