Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells of Aspergillus Awamori and Trichosporon Cutaneum

ABSTRACTTwo immobilized systems of Aspergillus awamori NRRL3112 and Trichosporon cutaneum R57 on modified polyamide beads were obtained. The optimal conditions for immobilization of strains Aspergillus awamori NRRL3112 and Trichosporon cutaneum R57 on polyamide beads were determined. It was found that by combined action of two immobilized systems it is possible to degrade up to 1.5 g·L−1 phenol, while individually immobilized and free cells did not have this capacity (degrade up to 1 g·L1). The high potential possibility and the synergistic action of both immobilized systems were proved. Biodegradation of different phenolic derivatives was investigated - 2\-chlorophenol (2-CP), 3-chlorophenol (3-CP), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), 4-n-nonylphenol (4-NP), 3-methoxyphenol (3-MP), bisphenol A (BPA), 4-chloro-3,5-dimethylphenol (chloroxylenol—ChX), and 2-benzyl-4-chlorophenol (chlorophen—ChP). It was found that the utilization of phenol, BPA, and 2,4-DCP was very fast (16 h); 2-CP, 3-CP,...