An ultra HPLC - mass spectrometry blood sugar and blood pressure lowering drugs method

The present invention provides an ultra-HPLC - mass spectrometry method for detection of blood 31 kinds of common hypotensive, hypoglycemic agents, characterized in that, said method comprising the steps of: (1) confirmation of the target compounds; (2) sample treatment: protein precipitation method using an organic solvent treated sample; (3) screening test: using ultra performance liquid chromatography - multiple reaction monitoring (MRM) tandem mass spectrometry (UPLC-MS / MS) mode of divided time segments screening detection; (4) the step of screening samples test positive (3) for review: screening test for positive samples, after treatment concentrating UPLC-MS / MS detection; optionally, and (5) step (4) review of the retest samples test positive, you can be employed HPLC - High Resolution time of flight tandem mass spectrometry (HPLC-QTOF) for review. According to the present invention each drug in the present invention is set detection conditions, the presence of different retention times, and thus completed the original segment detecting method. The present invention detects the total time 31 kinds of drugs may be required to complete in less than 11 minutes.