[A non-damaging procedure for DNA isolation from insect cell nuclei].
1982
: A gentle procedure is described for DNA isolation from Galleria mellonella cell nuclei. Nuclei lysis was performed by SDS in final concentration of 3% which was followed by DNA extraction from chromatin by a stepwise rise of NaCl concentration up to 2.5 M. After removal of nuclei insoluble components by centrifugation the native DNA was purified using the hydroxyapatite column. The method proposed provides a higher yield of the native DNA as compared with the phenol-detergent method.
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