Japanese encephalitis virus immunoglobulin M antibodies in porcine sera.

Abstract : A solid-phase enzyme-linked immunosorbent assay (ELISA) was developed for detection of porcine immunoglobulin (Ig)M antibodies to Japanese encephalitis virus (JEV). Antibodies in sera were captured onto the solid phase of Microtiter plates sensitized with mouse monoclonal antibodies to porcine u heavy chain. Virus antigen binding to the lawn of IgM as quantitated by subsequent binding of peroxidase-labeled human hyperimmune anti-JEV IgG, which, in the final step, catalyzed a substrate color change.