A simple andsensitive methodfordetermining transcription initiation site: identification oftwotranscription initiation sites inratgroup11phospholipase A2gene

We developed a simpleandsensitive methodfor assigning transcriptional initiation sites, andapplied it tocharacterize thetranscriptional unitofratgroup11 phospholipase A2(PLA2) gene.Ourmethodinvolves theprimer extension reaction followed bydetection of itsproducts byhybridization. Usingthismethod, we wereable tomaptwotranscriptional initiation sites on thenucleotide sequence ofthecorepromoter region ofPLA2genewithone-base resolution without any