며느리배꼽 잎 유래 캘러스의 부정근 형성에 미치는 지베렐린의 작용

This study was carried out to investigate the action of phytohormones which influence the adventitious root formation of calli originating from the leaves of Persicaria perfoliata. The optimal medium condition for callus formation was ½-strength MS, 1% sucrose, and 4.5 μM 2,4-D. In order to determine which phytohormones had an effect on the adventitious root formation, the calluses were cultured in various media with different kinds of phytohormones. As a result, the medium with GA3 or IAA was shown to induce root formation. To deeply investigate the effects of GA 3 and IAA, calli were cultured in 0.1, 1, and 10 mg/l levels of phytohormones. Numbers of roots formed per callus were 10.9, 14.2, 22.6 in GA3, 5.8, 3.9, 1.1 in IAA, respectively. Therefore, the higher GA 3 or the lower IAA concentration, the more roots formed. To confirm this role of GA 3 we tested with inhibitors PBZ and NPA. GA 3 with PBZ resulted in reduction by 52.4~69.4% compared to GA 3 alone. In contrast, GA 3 with NPA resulted in an increase by -8~45.6% compared to GA 3 alone in root formation. Also, results were determined on the effect of GA 3 with other phytohormones on root formation. Kinetin, 2iP and ABA with GA 3 had a negative effect, but IAA with GA 3 showed a similar result to GA 3 alone. From these results we infer GA plays a key role and auxin has subsidiary activity on adventitious root formation. This is the first report that indicates GA 3 promotes adventitious root formation from calli in P. perfoliata.