The effects of Fix&Perm reagents on flow cytometry detection for Th1/Th2/Th17 cell subsets

This study designed to explore the effects of different FixPthe percentage of IL-17 + Th cells stained by the improved FixPthere was no significant difference in the percentages of IFN-γ + Th cells stained by the three Fix&Perm reagents（P>0.05）.The results revealed that IFN-γ + Th,IL-4 + Th and IL-17 + Th cells could be detected by flow cytometry using the three Fix&Perm reagents,but different Fix&Perm reagents may affected the detection efficiency.So we should choose the optimized Fix&Perm reagent according to experimental purpose.