Bend-Insensitive Through-Fiber Stimulated Emission Depletion (STED) Imaging of HeLa Cells

We demonstrate, for the first time, STED images of biological samples where fluorescence has been collected through the same fiber used to transport excitation and depletion light (through-fiber imaging). We also quantitatively demonstrate fiber-bending-independent resolution. These results show promise for endoscopic and in vivo super-resolution imaging.