A method for determining the concentration of FG020326 in mice plasma by RP-HPLC

Aim To establish a method to determine the concentration of FG020326 in the plasma of KM mice by reverse phase high-performance liquid chromatography. Methods KM mice were used throughout the experiment and their body weights ranged from 20.0 to 25.1 g. All of them were distributed randomly to different test groups which each had 6 mice. FG020326 was administered at a dose of 30 mg·kg~ -1 via tail vein. The external standard method was used for the quantitation of FG020326. Results Under the condition of HPLC, the retention time of FG020326 was 7.9 min. The standard curve was =0.0144X-0.0285(r=0.9998) with a validated quantitation range of 162.5～ 4 1600 μg·L~ -1 , and the lowest concentration of detection in mice plasma was 40 μg·L~ -1 (S/N=3). At the concentration of 650、 2 600 、 20 800 μg·L~ -1 , absolute recovery rates were ranged from 68.66% to 84.63% and relative recovery rates were ranged from 92.58% to 110.88%. The RSD of the precision within-day and between-day were less than 3.2%. At ambient temperature and four freeze-thaw cycles, FG020326 was stable and the RSD was less than 1.3% and 6.0%, respectively. The concentration-time curve of FG020326 was well fitted to a two-compartment model. T_ [SX(]12α and T_ [SX(]12β were 0.088 h and 5.33 h, respectively. AUC_ 0→∞ was 14 183 h·μg·L~ -1 and V_d was 0.37 L. Conclusion The assay method is shown to be sensitive, suitable and accurate for determination of FG020326 in mice plasma.