Antitesticular activity of DL-6-(N-2-Pipecolinomethyl)-5-Hydroxy-Indane maleate (PMHI) in coyotes (canis latrans)☆☆☆

Abstract The ban on toxicants for coyote control has created a need for alternative control methods. Reproductive inhibitors may provide a means of maintaining coyote populations at levels where impact on live-stock is acceptable. Therefore, the effects of PMHI on testicular activity of coyotes were evaluated. Twelve mature, male coyotes were weighed and allotted randomly to 1 of 4 treatment groups: (1) controls, (2) 5, (3) 10, and (4) 20 mg PMHI/kg body weight (BW). PMHI was dissolved in 2 ml distilled water and ethanol (1:1) and administered as a single sc injection. All coyotes were observed for 1 hr after treatment and any adverse effects recorded. Fifteen days after treatment, a testicle and epididymis from each coyote was selected at random, ablated, weighed, fixed in formalin, and prepared for histologic examination. The remaining testicle was prepared similarly 30 days post-treatment. Data were analyzed by a balanced factorial in a split-plot arrangement. Unlike controls, coyotes given PMHI exhibited signs of nausea and diarrhea within 15 to 30 min, which subsided after 45 to 60 min. PMHI temporarily increased (P