The roles of sympathetic nerve on embryo implantatio and local immunity in uterus of mice

To explore the effect mechanism of sympathetic nerve on early developing embryo of mice. The model of sympathectomy mouse was established by 6-OHDA injected intraperitoneally. The early developing mice embryo and lymphocytes in uterus were observed using histology, immunohistochemistry and ELISA methods. In sympathectomy mouse, the number of embryo implantation was decrease to 64.4 %, endometrial lamina propria and vaseellum were shortfall .The numbers of CD4~(+) T cell and CD8~(+) T cell in sympathectomy mice were larger than ones in control groups, especially, the number of CD8~(+) T cells at E3 and E5 differed highly （P<0.01）. The quantity of IL-2 in sympathectomy mouse increased, and it was the highest at ES, increased to 3.6 times. The quantity of IL-4 was higher than that of control mouse only at ES, and the IL-2/IL-4 increased in sympathectomy mouse uterus. Changes of uterus histology and CD4~(+), CD8~(+)T cells and Th1/Th2 cells are the ones of effects of sympathetic nerve on early embryo development.