Supplemental Data Global Regulators Orchestrate Group II Intron Retromobility

Plasmid copy number (PCN) was determined after induction of the donor intron. Total genomic and plasmid DNA was isolated using the QIAGEN QIAamp DNA kit according to the manufacturer’s protocol. Standard quantitative PCR conditions, using SYBER green master mix (Applied Biosystems), were then used as described in Supplementary Table S2 by (Coros et al., 2008). A standard curve of fluorescence versus cycle number (C