Characterization of a Thermostable Endoglucanase from Cellulomonas fim i ATCC484

Bacteria in the genus Cellulomonas are well known as secretors of a variety of mesophilic carbohydrate degrading enzymes (e.g. cellulases and hemicellulases), active against plant cell wall polysaccharides. Recent proteomic analysis of the mesophilic bacterium Cellulomonas fimi ATCC484 revealed uncharacterized enzymes for the hydrolysis of plant cell wall biomass. Celf_1230 (CfCel6C), a secreted protein of Cellulomonas fimi ATCC484, is a novel member of the GH6 family of cellulases which could be successfully expressed in Escherichia coli. This enzyme displayed very little enzymatic/hydrolytic activity at 30oC, but showed an optimal activity around 65 o C and exhibited a thermal denaturation temperature of 74 o C. In addition, it also bound strongly to filter paper despite having no recognizable carbohydrate binding module. Our experiments show that CfCel6C is a thermostable endoglucanase with activity on a variety of β-glucans produced by an organism that struggles to grow above ...