Pulse electric field effect on cervical cancer cell: a plant based anti-cancer study
2018
Cancer is listed in top ten global causes of death in 2016. The percentage of cancer
incidence and mortality are steadily rising over the year. This necessitates alternate
anti-cancer treatment as the conventional treatment have resulted in high number of
long- term survivors who are left to deal with the aftereffect of their therapy. Drug
delivery is a challenging aspect in medical community as the plasma membrane is
non-permeable to external molecules. From this context, a study was proposed to use
of natural plant extract Artocarpus altilis which will be uploaded into cervical cancer
cell with the aid of electrical pulses or electroporation technique to investigate on the
anti-cancer mechanism. Electroporation parameters utilized in this study ranging
from 200V/cm, 400V/cm, 600V/cm, 800V/cm, 1000V/cm of pulse amplitude, while
pulse duration ranging from 100μs, 500μs, 1ms, 5ms and 10ms with single pulse.
Three parts of artocarpus altilis tested in this research study which is the peel, pulp
and whole fruit. The concentration of all three parts which were tested on cervical
cancer cell ranged from 10μg/ml, 20μg/ml, 30μg/ml, 40 μg/ml, and 50μg/ml with
methanol as the solvent. It is found that pulse electric field increased the cell
spreading up to 129.8% with 600V/cm of pulse amplitude, which stimulates to the
highest cell length percentage. Apart from that, cell viability found to be decrease
with an increase in pulse amplitude and pulse duration. 600V/cm of pulse amplitude
and 10ms of pulse duration stimulates highest cell viability with 53.3% as compared
to control group (without treatment). Cell viability of cervical cancer cell measured
when cell subjected to electrical field alone, extract alone and both electrical field
and extract and the result of the study revealed that lowest cell viability stimulated by
the combination of electrical pulse and extract up to 28% of cell viability. Thus the
outcome of this research revealed new targets and electrochemical pathways for
cervical cancer treatment while with the discovery health of patients will be
maintained and there will be an improvement in general life expectancy.
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