Effect of benazepril on oxidative stress and carbonyl stress in renal tissues of diabetic rats

Objective To investigate the effects of Benazepril on oxidative and carbonyl stress in diabetic rat kidneys, and to explore the renoprotective mechanisms in diabetic rats. Methods Rats were randomly divided into three sub-groups, including, normal control (group A) , diabetic control (group B) and diabetic with Benazepril (group C). Blood glucose, HbAlC, the ratio of kidney to body weight and urinary protein excretion were measured. Malonyldialdehyde (MDA) level, superoxide dismutase (SOD) activity, glutathione peroxidase (CSH-Px) activity, total sulfhydryl group and protein carbonyl levels in renal tissue were also determined. Result Compared to group A, the ratio of kidney to body weight, urinary protein excretion, MDA and protein carbonyl levels in group B were significantly higher, while SOD,GSH-Px activities and total sulfhydryl group level were significantly lower. The ratio of kidney to body weight, urinary protein excretion, MDA and protein carbonyl level in group C were significantly lower than those in group B. GSH-Px activity in group C were higher than that in group B. Conclusion Benazepril can prevent renal hypertrophy and decrease urinary protein excretion in diabetic rats. The renoprotective effects of this drug may be related to the ability to inhibit oxidative and carbonyl stress. Key words: Benzazepines/PD; Diabetes mellitus;  experimental/PA; Oxidative stress/DE