PARTICLES AS PROTEIN MARKERS: NANOSCALE MICROSCOPY TOWARDS PICOSCALE

We proposed the proteins-nanogel particles as potential nanogel adducts for their commercial value in measurement of enhanced metabolic energy mechanism by muscle contractile protein conformational changes at picoscale. Picoscale (at the down-side level of 10 -12 ) is the minutest limit of molecular detection till date. In muscle, tropomyosin-calcium bound nanogels trigger the conformational changes at picoscale (10 -12 meters or 10 -2 °A) and offer a promise of safe and rapid modality to increase the energy holding capacity of muscle tropomyosine-calcium protein assembly. Till date, electron microscopy and biophysical techniques can predict these submolecular physical dimensions without any information of metabolic energy mechanism or dynamicity. The proposed picoscale measurement of in vivo protein concentration and molecular dynamic events in Heteropneustes fossilis fish muscle may open a window to predict subphysiological, submolecular conformation to design hyperenergetic marine diets to get high quality fish food. The present report shows the emerging trend of picotechnology in bioengineering, Heteropneustes fossilis fish protein characterization by PAGE-gel electrophoresis, 10-100 nm nanoadducts by electron microscopy with possibility of nanogel-peptide(polyacylate-polyethylene-iron oxide) adducts as predictors of fish muscle proteins participating to enhance the capacity of tropomyosin-calcium hyperexcitation to measure it at picoscale in muscle tissue. In conclusion, the nanogel-protein adduct as bio-technology has tremendous commercial potentials to design marine diets, aquaculture, nanogel-drug carriers.