A luminol electrochemiluminescence aptasensor based on glucose oxidase modified gold nanoparticles for measurement of platelet-derived growth factor BB.

Abstract A sandwich-type luminol electrochemiluminescence (ECL) aptasensor for highly sensitive and selective detection of platelet-derived growth factor BB (PDGF-BB) is fabricated. For this proposed ECL aptasensor, a multilayered AuNPs-electrochemically reduced graphene (AuNPs-EG) nanocomposite film was formed on the GCE surface as the base of the aptasensor through a co-electrodeposition method. The AuNPs-EG composites possess high conductivity to promote the electron transfer at the electrode interface and good biocompatibility and large surface area to capture large amounts of primary aptamer (Apt1), thus amplifying the detection response. Moreover, glucose oxidase (GOD) functionalized AuNPs labeled secondary aptamer (GOD–Apt2–AuNPs) was designed as the signal probe for the sandwiched aptasensor. Enhanced sensitivity was obtained by in situ generation of H 2 O 2 from reaction between GOD and glucose and the excellent catalytic behavior of AuNPs to the ECL of the luminol–H 2 O 2 system. Under the optimal conditions, the as-prepared ECL aptasensor exhibited excellent analytical property for the detection of PDGF-BB in the range from 1.0×10 –13 to 5.0×10 −10  mol L −1 with a detection limit of 1.7×10 –14  mol L −1 ( S / N =3). The application of the present protocol was demonstrated by analyzing PDGF-BB in human serum and human urine samples with the recoveries from 85.0% to 110%.