EVALUATION OF POLYMORPHISM OF IL-10 IN CHILDREN WITH GINGIVITIS

Introduction: Gingivitis is a multifactorial disease in which host immune system and genetic factors have an important role in its pathogenesis. Genetic polymorphisms in cytokines and their receptors have been proposed as potential markers for periodontal disease. Recent studies have suggested IL-10 gene polymorphism to be involved with gingivitis in children. The aim of this study was to evaluate IL-10 gene polymorphism association with gingivitis in 8-12 year old children. Materials & Methods:In this case-control study, approved by Hamadan University of Medical Sciences, 100 children were divided into two groups of 50 controls and cases, according to the periodontal indices. An epithelial sample from buccal mucosa of each individual was prepared and DNA was extracted by Miller's salting out method. Then mutation at position -1082 (H/L), -819 (C/T) in the IL-10 gene was detected by PCR-RFLP method and the data were analyzed by chi-square test. Results:Results showed that at position-1082, distribution of H allele in case group was 34 and for control group it was 24 (P=0.27). Distribution of L allele in two groups was 98 (P=1). Callele distribution in-819 position in case group was 36 and for the control group was 42 (P=0.54) and T allele distribution for case group was 94 and for control group was 90 (P=0.71). There was no significant difference in genotype distribution between the groups. Conclusion:This study suggests that there is no correlation between IL-10 polymorphism with gingivitis in 8-12 year old children.