COMPOSITION OF THE SOLUBLE, NONDIALYZABLE COMPONENTS IN RAW CANE SUGAR

The high molecular weight (HMW) material (> 12, 000 Da) often raw cane sugars from seven countries and two U.S. states was isolated by dialysis and analyzed by gel permeation chromatography (GPC). Simultaneous detection of colorant polymers and polysaccharides was accomplished by using two detectors in series - UV at 214 nm for colorant polymers and refractive index (RI) for carbohydrate polymers. The monosaccharide composition of the nondialyzable raw sugar fraction (the tenate) was determined by hydrolysis with trifluoroacetic acid and with oxalic acid. Aconitic acid was associated with the tenate. The raw sugars were categorized into two types according to their GPC patterns and aconitic acid content. Hydrolysis of individually collected GPC peaks demonstrated that one particular peak contained most of the aconitic acid. Le sucre brut est un sucre non comestible, de couleur brune et a gros cristaux produit a partir du jus extrait de la tige de la canne a sucre. L'objectif de cette etude est de determiner le profil de composition et de poids moleculaires des composes a haut poids moleculaire (>12000 Da) du sucre brut. Les colorants et les polyosides sont examines dans 10 sucres bruts, representant les lieux de production dans le monde. La composition en oligosides de la portion soluble, non dialysable est determinee par hydrolyse acide, et le profil en poids moleculaire des composes a haut poids moleculaires est determine par chromatographie par permeation de gel.