Structure and Function Analysis of SigL and Its Enhancer-binding Proteins in Bacillus thuringiensis Strain YBT-1520
2012
To reveal the regulatory functions of SigL and its enhancer-binding proteins(EBPs) in Bacillus thuringiensis,the structure and function of SigL and its EBPs of YBT-1520 strain were in depth analyzed through bioinformatics methods after the whole genome sequencing.The results indicated that there were one and six coding genes for SigL and EBPs in YBT-1520 genome,respectively.Furthermore,the EBPs displayed rich structural diversity and possessed all organization types of EBPs domains.The genes regulated by SigL should be related with 11 putative COG metabolism pathways,including energy metabolism,amino acid metabolism,translation and cell cycle.According to the location of EBPs in YBT-1520 genome,some EBPs were predicted to regulate the metabolism pathways of γ-aminobutyric acid and arginine,and the degradation of branched chain fatty acid and polysaccharide.This study will provide a new research direction on the regulatory mechanisms for high expression of insecticidal crystal proteins.
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