The effects of TSA combined with HSV-1 on proliferation and apoptosis of U251 cells

Objective To observe the effects of trichostain A （TSA） combined with herpes sim- plex virus-1 （HSV-1） on proliferation and apoptosis in U251 cells in vitro. Methods U251 ceils in vitro were treated separately with 0.5×10^-3μmol/Lmol/L TSA （TSA group）, 10 MOI HSV-1 （HSV-1 group）, and 0.5×10^-3μmol/L TSA combined with 10 MOI HSV-1 （TSA ＋ HSV-1 group） for 72 h. The methyl thia- zol tetrazolium （MTT） assay was used to observe the proliferation of U251 cells. The cellular morphological changes were observed under a ruicroscope. The apoptosis rate was analyzed by using flow cytometry. Resuits In TSA group, HSV-1 group, TSA ＋ HSV-1 group, the proliferation inhibition rate of the U251 cells was （48.0±1.8）%, （18.0±3.1）%, and （58.0±5.8）%; Apoptosis rate was （47.4±3.5）%, （29.6±3.0） %, and （ 59. 6±4. 0 ） % respectively. The inhibitory effects of proliferation or apoptosis in TSA± HSV-1 group was stronger than TSA group or HSV-1 group （ P 〈 0. 01 ）. Conclusion TSA combined with HSV-1 has a synergistic killing effect on cultured U251 cells. Key words: Trichostain A ;  Herpes simplex virus-1 ;  Glioma ;  Proliferation ;  Apoptosis