The Effect of Benfothiamin and Vitamin D in Ischemia / Reperfusion Model of Rat Skeletal Muscle -

Objects: Benfothiamin is a highly potent form of vitamin B1 protecting endothelial function. On the other hand, vitamin D provides restoration of muscular tissue by inhibition of apoptosis and acceleration of cellular proliferation following muscle injury. We assumed that the administration of these vitamins in ischemia/ reperfusion (I/R) injury, could reduce the damage by alteration of the release of various oxidant and antioxidant mediators leading to cellular damage. Materials and Methods: We assigned 30 Wistar Albino males rats into 5 groups. In the control group (n=6), rats were anaesthetized and total antioxidant capacity (TAS), total oxidant capacity (TOS), malondialdehyde (MDA), superoxide dismutase (SOD) and nitricoxide (NO) level were measured in lower extremity soleus muscle. Benfotiamin and D were given to the groups and the values of these parameters were evaluated in ischemia reperfusion muscle tissue specimens. All tissues were examined histologically. Results: We detected a significant change in groups 3 and 4 for antioxidant NO level after ischemia and reperfusion. Therefore, we observed that the administration of vitamin D and benfothiamin increased NO levels in muscle especially during reperfusion. The level of other oxidants TOS and MDA and antioxidants TAS and SOD were not significant during I/R at given periods. Overall vitamin D and benfothiamin have acute beneficial effects especially in improving I/R injury of lower extremity, even at non-critical periods. Conclusion: Acute term effects of benfothiamin and vitamin D can be useful during where changes due to I/R. The effects can be evaluated during long term I/R.