Expression of Physiologic Biomolecules following Anti - Motif Oligodeoxynucleotide

According to latest estimates, there were 198 million cases of 2013, with 82% of these cases occurring in Africa. There were 584,000 malaria deaths worldwide. The outcome of parasites is determined by the activities of various biomolecules, cytokines and other host-specific factors. Plasmodia parasites evade immun immune system s to their advantage thereby exacerbating When combined in therapy, immunostimulatory unmethylated CpG motif oligodeoxynucleotides (ODNs) synergise with cytokines parasitic mechanisms providing effective protection in ODN s enhance immune activities through ligation to (pDC) Toll-like receptors (TLRs) such as TLR-9 and they activate both cells, while cytokines modulate cellular behaviour functionality, the cytokine -CpG ODN immunotherapy combination expression of physiologic factors and this can influence disease severity of infection with malaria parasites. It was previously physiologic biomolecules like m atrix metalloproteinases (MMPs) and angiopoietins (ANGPTs) could be influenced by the coincidental introduction of recombinant cytokines and CpG ODNs during malaria. This project studied ODN co -inoculation in BALB/c mice infected with P. berghei BALB/c mice groups infe cted with virulent blood stage parasites were given immunotherapeutic cytokine and CpG ODN combinations for five consecutive days while six other control groups with different tre included for comparison . The mice were monitored daily for parasitaemia development from day 1 postinfection. At mice were humanely sacrificed for the extraction of EDTA for measuring various physiologic factors . Results unraveled gene therapy as an enhancer of anti-Plasmodial activities in adiponectin, ANGPT1, neuropilin-1(NRP-1) and cyclooxygenase delevations in ANGPT2, MMP -8 and MMP-9. These physiologic outcomes, which are largely agreeable with data from other studies, favour further investigation combinatorial cytokine-CpG ODN gene therapy for potential inclusion into preventative and therapeutic anti -malarial interventions.